CD58 antibody

Catalog No. ABIN118746

This anti-CD58 antibody is a Mouse Monoclonal antibody detecting CD58 in FACS and WB. Suitable for Human.

Quick Overview for CD58 antibody (ABIN118746)

Target: CD58

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD58 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Clone: BRIC-5

Product Details anti-CD58 Antibody

Specificity: BRIC5 was produced in response to erythrocytes. It reacts by Immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.Aff. Const.:4 x 10e8 M-1

Purification: Affinity Chromatography on Protein G.

Immunogen: Human erythrocytes.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD58

Target: CD58

Alternative Name: CD58 / LFA3

Background: CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 Molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Synonyms: Ag3, LFA-3, Lymphocyte function-associated antigen 3

Gene ID: 965

NCBI Accession: NP_001138294

UniProt: P19256